human hnscc cell lines scc15  (ATCC)

Journal: Cell Death Discovery

Article Title: USP25 attenuates the immunosuppressive tumor microenvironment via the deubiquitination of TAB2 in head and neck squamous cell carcinoma

doi: 10.1038/s41420-025-02883-1

Figure Lengend Snippet: A A model of oral carcinogenesis was established in mice by 4-NQO drinking water. B Representative images of tongue specimens from mice collected at weeks 16, 26, and 32. C The statistical graph shows the percentage of patients with lymph node metastasis. D HE staining of tumor tissues and normal tissues. E The results of IHC staining show the levels of USP25 in the indicated groups. F The expression of USP25 in normal tissue, dysplasia tissue, and tumor tissue are shown. G Western blot analysis of the SCC15 and SCC25 cell lines. H CCK8 assays for the SCC15 and SCC25 cell lines. I Wound-healing assays for HNSCC cells overexpressing USP25. J Transwell assays for HNSCC cells with USP25 overexpression. Data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: HNSCC cell lines SCC15 and SCC25 were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Staining, Immunohistochemistry, Expressing, Western Blot, Over Expression

Journal: Cell Death Discovery

Article Title: USP25 attenuates the immunosuppressive tumor microenvironment via the deubiquitination of TAB2 in head and neck squamous cell carcinoma

doi: 10.1038/s41420-025-02883-1

Figure Lengend Snippet: A Volcano plot of the RNA-seq data (red and blue indicate significantly upregulated and downregulated genes, respectively). B Western blot analysis of IL-6 expression in HNSCC cells with USP25 downregulation or overexpression. C ELISA results showing the changes in IL-6 secretion in cell supernatants. D In vitro chemotaxis assay for MDSCs cocultured with SCC15 and SCC25 cells. Scale bar, 100 μm. E KEGG enrichment analysis revealed that MAPK signaling was significantly enriched. F The abundance of c-Jun, P-p38, p38, P-ERK, and ERK was measured by western blotting. Data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: HNSCC cell lines SCC15 and SCC25 were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: RNA Sequencing, Western Blot, Expressing, Over Expression, Enzyme-linked Immunosorbent Assay, In Vitro, Chemotaxis Assay

Journal: Cell Death Discovery

Article Title: USP25 attenuates the immunosuppressive tumor microenvironment via the deubiquitination of TAB2 in head and neck squamous cell carcinoma

doi: 10.1038/s41420-025-02883-1

Figure Lengend Snippet: A Proteins were immunoprecipitated from cell lysates with anti-USP25 antibody or anti-TAB2 antibody ( B ) and then analyzed by immunoblotting. C Immunofluorescence of USP25 (red) and TAB2 (green) in HNSCC cells. Scale bar, 20 µm. D Schematic domain structure of USP25 and different mutants. E IP and immunoblot analysis of HEK293T cells transfected with the indicated plasmids. F HEK293T cells were transfected with the indicated plasmids, and proteins were immunoprecipitated with anti-HIS antibody and analyzed by immunoblotting. G The polyubiquitination level of endogenous TAB2 in SCC15 and SCC25 cells stably expressing USP25, catalytically inactive mutant of USP25 (H608A), was measured by an in vitro deubiquitination assay. Data represent the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: HNSCC cell lines SCC15 and SCC25 were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Immunoprecipitation, Western Blot, Immunofluorescence, Transfection, Stable Transfection, Expressing, Mutagenesis, In Vitro

Journal: Cell Reports Medicine

Article Title: Co-targeting BMI1 and MYC to eliminate cancer stem cells in squamous cell carcinoma

doi: 10.1016/j.xcrm.2025.102077

Figure Lengend Snippet: PTC209-induced DNA damage activates NF-κB signaling pathway in CSCs (A) Immunostaining and quantification of pH2A.X (red) in HNSCC cells treated with PTC209. DAPI stained the nuclei blue. Scale bar, 20 μm. (B) Percentage of pH2A.X in HNSCC cells from different treatment groups as indicated. Data are shown as the mean ± SD. ∗∗ p < 0.01 using an unpaired Student’s t test. (C) Western blot analysis of pH2A.X in HN6 and SCC15 cells treated with PTC209. (D and E) Histogram of the top 10 most-enriched GO (D) and KEGG (E) terms for upregulated genes (FC > 2) in ALDH + HN6 cells treated with PTC209. The red rectangles indicate genes related to NF-κB signaling. (F) Western blot analysis of pIKK-α/β, IKK-α, IKK-β, pIκB-α, IκB-α, p-P65, and P65 in HNSCC cells with PTC209 treatment. (G) Representative images of p65 immunostaining (green) in HN6 and SCC15 cells treated with PTC209. DAPI stained the nuclei (blue). Scale bar, 20 μm. See also .

Article Snippet: The human HNSCC cell line SCC15 was from the American Type Culture Collection (ATCC, Manassas, VA, USA), and HN6 was from the Central Laboratory of Peking University School and the Hospital of Stomatology (Beijing, China).

Techniques: Immunostaining, Staining, Western Blot

Journal: Cell Reports Medicine

Article Title: Co-targeting BMI1 and MYC to eliminate cancer stem cells in squamous cell carcinoma

doi: 10.1016/j.xcrm.2025.102077

Figure Lengend Snippet: IL-6 secreted from CSCs is responsible for non-stem tumor cells dedifferentiation (A) Heatmap of RNA-seq data for expression of genes encoding proinflammatory cytokines in ALDH + HN6 cells treated with PTC209. (B) Real-time qPCR analysis of IL-6 mRNA expression in HN6 and SCC15 cells treated with PTC209. Data are shown as the mean ± SD. ∗ p < 0.05 and ∗∗ p < 0.01 using an unpaired Student’s t test. (C) ELISA of protein levels of IL-6 secreted by HN6 and SCC15 cells treated with PTC209. Means ± SD are shown. ∗∗ p < 0.01 using an unpaired Student’s t test. (D) Real-time qPCR analysis of MYC in HN6 and SCC15 cells treated with IL-6. Data are shown as the mean ± SD. ∗∗ p < 0.01 using an unpaired Student’s t test. (E) Western blot analysis of MYC in HN6 and SCC15 cells treated with IL-6. (F) Experimental design used to label Bmi1 + CSCs after PTC209 plus IL-6 inhibitor treatment in mouse HNSCC tumors. (G) Representative images of mice with Bmi1 + Tomato + CSCs in HNSCC tumors from different treatment groups as indicated. The white dotted line indicates the boundary between tumor and interstitial tissues. Scale bar, 20 μm. (H) Percentage of Bmi1 + Tomato + CSCs in HNSCC tumors from different treatment groups as indicated. Data are shown as the mean ± SD. n = 5. ∗∗ p < 0.01 using one-way ANOVA. See also .

Article Snippet: The human HNSCC cell line SCC15 was from the American Type Culture Collection (ATCC, Manassas, VA, USA), and HN6 was from the Central Laboratory of Peking University School and the Hospital of Stomatology (Beijing, China).

Techniques: RNA Sequencing, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot